Single molecule manipulation using magnetic tweezers

Work carried out as part of a CDD at the CNRS in the ABCD Lab team. The task was to prepare and install treated microfluidic channels to fix DNA strands. The DNA strands were equipped with a metal bead at one end, which allows for the application of a force (a few pN) to stretch the polymer. These strands were designed to form G-4 complexes and hairpins. Under certain conditions, polymerase enzymes can unfold and break G-4. I was able to study the influence of flavoids on polymerase activity.